The HLDA8 blind panel: Findings and conclusions

Swart B., Salganik M., Wand M., Tinckam K., Milford E., Drbal K., ...Daha Fazla

JOURNAL OF IMMUNOLOGICAL METHODS, cilt.305, sa.1, ss.75-83, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 305 Sayı: 1
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.jim.2005.07.003
  • Dergi Adı: JOURNAL OF IMMUNOLOGICAL METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.75-83
  • Hacettepe Üniversitesi Adresli: Evet

Özet

There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. (c) 2005 Elsevier B.V. All rights reserved.