A novel affinity support material for the separation of immunoglobulin G from human plasma


Garipcan B., Denizli A.

MACROMOLECULAR BIOSCIENCE, cilt.2, sa.3, ss.135-144, 2002 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 2 Konu: 3
  • Basım Tarihi: 2002
  • Dergi Adı: MACROMOLECULAR BIOSCIENCE
  • Sayfa Sayıları: ss.135-144

Özet

2-Methyacrylamidohistidine (MAH) as a pseudospecific ligand was synthesized from methacryl chloride and histidine. Spherical beads with an average size of 50-63 mum were obtained by the radical suspension polymerization of MAH and 2-hydroxyethyl methacrylate (HEMA) conducted in an aqueous dispersion medium. Owing to the reasonably rough character of the bead surface, P(HEMA-co-MAH) beads had a specific surface area of 17.6 m(2) (.) g(-1). Synthesized MAH was characterized by NMR. P(HEMA-co-MAH) beads were characterized by selling studies, FT-IR spectroscopy, scanning electron microscopy (SEM) and elemental analysis. P(HEMA-co-MAH) affinity beads with a swelling ratio of 65% were used in the separation of human immunoglobulin G (HIgG) from aqueous solutions and human plasma. The maximum HIgG adsorption on the P(HEMA-co-MAH) adsorbents was observed at pH 7.4 for phosphate and at pH 6.0 for morpholinoethanesulfonic acid buffers. The HIgG adsorption onto the PHEMA adsorbents was negligible. Higher adsorption values (up to 46.5 mg (.) g(-1)). Adsorption capacities of other blood proteins were obtained as 3.2 mg (.) g(-1) for fibrinogen and 4.6 mg (.) g(-1) for albumin. The total protein adsorption was determined to be 82.2 mg (.) g(-1). The pseudospecific affinity beads allowed one-step separation of HIgG from human plasma. HIgG molecules could be repeatedly adsorbed and desorbed with these adsorbents without noticeable loss in their HIgG adsorption capacity.