Biochemical-genetic comparison of Cobitis simplicispina from Turkey, and C. taenia, C. albicoloris and C. elongata from Bulgarian freshwaters

Ivanova P., Erk'akan S., Ozeren S., Dobrovolov I.

FOLIA BIOLOGICA-KRAKOW, vol.51, pp.79-84, 2003 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 51
  • Publication Date: 2003
  • Journal Name: FOLIA BIOLOGICA-KRAKOW
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.79-84
  • Hacettepe University Affiliated: No

Abstract

Taxonomic investigations of 4 species of the genus Cobitis: C. simplicispina Hanko 1925, C. taenia Linnaeus 1758, C. albicoloris Chichkoff, 1932 (= C. peshevi Sivkov & Dobrovolov, 1984) and C. elongata (Heckel & Kner 1858) from Turkey and Bulgarian freshwaters were carried out using biochemical-genetic methods. The analyses of one general muscle protein (PROT) and six enzyme systems including esterase (EST), lactate dehydrogenase (LDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), malic enzyme (MEP), malate dehydrogenase (MDH) and superoxide dismutase (SOD) revealed electrophoretic differences between the species examined. Fractions controlled by the LDH-B locus of C. simplicispina gave completely different electrophoretic mobility from those of the other Cobitis species. Nei's genetic distance (D-Nei) between C. simplicispina and C. albicoloris, C. taenia and C. elongata was estimated as 1.39, 1.16 and 1.27, respectively. C. simplicispina might have diverged from the other three species 5.8 - 6.95 million years ago.