Methacryloylamidohistidine in Affinity Ligands for Immobilized Metal-ion Affinity Chromatography of Ferritin


AKTAŞ UYGUN D., Ozturk N., AKGÖL S., DENİZLİ A.

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, cilt.16, sa.1, ss.173-179, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 16 Konu: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s12257-009-0162-4
  • Dergi Adı: BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
  • Sayfa Sayıları: ss.173-179

Özet

A new metal-chelate adsorbent utilizing 2-methacryloylamidohistidine (MAH) was prepared as a metal-chelating ligand. MAH was synthesized using methacryloly chloride and histidine. Monosize nanospheres with an average diameter of 450 nm were produced by emulsion polymerization of 2-hydroxyetylmethacrylate (HEMA) and MAR Then, Fe3+ ions were chelated directly onto the monosize nanospheres. Mon-poly(HEMA-MAH) nanospheres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and elemental analysis. Fe3+ chelated monosize nanospheres were used in ferritin adsorption from an aqueous solution. The maximum ferritin adsorption capacity of Fe3+-chelated mon-poly(HEMA-MAH) nanospheres was 202 mg/g at pH 4.0 in acetate buffer. The non-specific ferritin adsorption on the monpoly(HEMA-MAH) nanospheres was 20 mg/g. The adsorption behavior of ferritin could be modeled using both Langmuir and Freundlich isotherms. The adsorption capacity decreased with increasing ionic strength of the binding buffer. High desorption ratios (> 95% of the adsorbed ferritin) were achieved with 1.0 M NaCl at pH 7.0. Ferritin could be repeatedly adsorbed and desorbed with the Fe3+-chelated mon-poly(HEMA-MAH) nanospheres without significant loss of adsorption capacity.