Dog liver glucose-6-phosphate dehydrogenase: purification and kinetic properties


Ozer N., Bilgi C., Ogus I.

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, cilt.34, sa.3, ss.253-262, 2002 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Konu: 3
  • Basım Tarihi: 2002
  • Doi Numarası: 10.1016/s1357-2725(01)00125-x
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
  • Sayfa Sayıları: ss.253-262

Özet

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first step of the pentose phosphate pathway which generates NADPH for anabolic pathways and protection systems in liver. G6PD was purified from dog liver with a specific activity of 130 U mg(-1) and a yield of 18%. PAGE showed two bands on protein staining; only the Slower moving band had G6PD activity. The observation of one band on SDS/PAGE with M-r of 52.5 kDa suggested the faster moving band on native protein staining was (lie monomeric form of the enzyme.