Dog liver glucose-6-phosphate dehydrogenase: purification and kinetic properties
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, vol.34, no.3, pp.253-262, 2002 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 34 Issue: 3
- Publication Date: 2002
- Doi Number: 10.1016/s1357-2725(01)00125-x
- Journal Name: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.253-262
- Hacettepe University Affiliated: Yes
Abstract
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first step of the pentose phosphate pathway which generates NADPH for anabolic pathways and protection systems in liver. G6PD was purified from dog liver with a specific activity of 130 U mg(-1) and a yield of 18%. PAGE showed two bands on protein staining; only the Slower moving band had G6PD activity. The observation of one band on SDS/PAGE with M-r of 52.5 kDa suggested the faster moving band on native protein staining was (lie monomeric form of the enzyme.