Cytotoxic effect of endodontic irrigants in vitro.
Medical science monitor basic research, cilt.20, ss.22-26, 2014 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 20
- Basım Tarihi: 2014
- Doi Numarası: 10.12659/msmbr.890247
- Dergi Adı: Medical science monitor basic research
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.22-26
- Anahtar Kelimeler: Sodium Hypochlorite - toxicity, Chlorhexidine - toxicity
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Hacettepe Üniversitesi Adresli: Evet
Özet
Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. The aim of this study was to assess the cytotoxic effect of NaOCl 3%, Chx 2%, and MTAD on rat periodontal ligament fibroblasts, at 0.1 and 100 μl/mL, using WST-1 colorimetric method. Rat ligamental fibroblasts were exposed to the irrigants and their viability was assessed after 1, 24, 48, and 72 h. The measurements were determined using WST-1 assay, using a micro ELISA reader. At 100 ml/L all 3 irrigants were strongly cytotoxic, although CHX was less so than NaOCl and MTAD. At the 0.1 ml/L concentration, NaOCl and MTAD were only moderately cytotoxic, whereas Chx was highly deleterious to cell viability at all time points. There was a significant influence of the dilution rate of the substance, because the odds ratio for cell viability being over 50% was increased 51 times between the 100 ml/L and 0.1 ml/L dilutions. It seems that irrigating solutions should be used at lower concentrations to enhance cell viability.