DNA authentication of Plantago herb based on nucleotide sequences of 18S-28S rRNA internal transcribed Spacer region


Sahin F. P. , YAMASHITA H., GUO Y., TERASAKA K., KONDO T., YAMAMOTO Y., ...Daha Fazla

BIOLOGICAL & PHARMACEUTICAL BULLETIN, cilt.30, ss.1265-1270, 2007 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 30 Konu: 7
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1248/bpb.30.1265
  • Dergi Adı: BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Sayfa Sayıları: ss.1265-1270

Özet

Internal transcribed spacer (ITS) regions of nuclear ribosomal RNA gene were amplified from 23 plant- and herbarium specimens belonging to eight Plantago species (P. asiatica, P. depressa, P. major, P. erosa, P. hostifolia, P. camtschatica, P. virginica and P. lanceolata). Sequence comparison indicated that these Plantago species could be identified based on the sequence type of the ITS locus. Sequence analysis of the ITS regions amplified from the crude drug Plantago Herb obtained in the markets indicated that all the drugs from Japan were derived from P asiatica whereas the samples obtained in China were originated from various Plantago species including P. asiatica, P. depressa, P. major and P. erosa.