Rapid Method for Quantitative Determination of Proteolytic Activity with Cyclic Voltammetry
ELECTROANALYSIS, cilt.22, sa.3, ss.265-267, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 22 Sayı: 3
- Basım Tarihi: 2010
- Doi Numarası: 10.1002/elan.200900344
- Dergi Adı: ELECTROANALYSIS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.265-267
- Hacettepe Üniversitesi Adresli: Evet
Özet
An alternative electrochemical method for the determination of proteolytic activity based on cyclic voltammetry of the electroactive probe ferricyanide (Fe(CN)(6)(3-)) on the gelatin film coated ITO electrodes was proposed. The gelatin film behaves as a kinetic barrier for Fe(CN)(6)(3-) and the proteolytic digestion of the gelatin film results an increase in the penetration of the Fe(CN)(6)(3-). Thus, by the change of peak potential in cyclic voltammogram of Fe(CN)(6)(3-), activity of the enzyme was determined.