Rapid Method for Quantitative Determination of Proteolytic Activity with Cyclic Voltammetry


Bas D., BOYACI İ. H.

ELECTROANALYSIS, cilt.22, sa.3, ss.265-267, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Konu: 3
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1002/elan.200900344
  • Dergi Adı: ELECTROANALYSIS
  • Sayfa Sayıları: ss.265-267

Özet

An alternative electrochemical method for the determination of proteolytic activity based on cyclic voltammetry of the electroactive probe ferricyanide (Fe(CN)(6)(3-)) on the gelatin film coated ITO electrodes was proposed. The gelatin film behaves as a kinetic barrier for Fe(CN)(6)(3-) and the proteolytic digestion of the gelatin film results an increase in the penetration of the Fe(CN)(6)(3-). Thus, by the change of peak potential in cyclic voltammogram of Fe(CN)(6)(3-), activity of the enzyme was determined.