Rapid Method for Quantitative Determination of Proteolytic Activity with Cyclic Voltammetry

Bas D., BOYACI İ. H.

ELECTROANALYSIS, vol.22, no.3, pp.265-267, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 3
  • Publication Date: 2010
  • Doi Number: 10.1002/elan.200900344
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.265-267
  • Hacettepe University Affiliated: Yes

Abstract

An alternative electrochemical method for the determination of proteolytic activity based on cyclic voltammetry of the electroactive probe ferricyanide (Fe(CN)(6)(3-)) on the gelatin film coated ITO electrodes was proposed. The gelatin film behaves as a kinetic barrier for Fe(CN)(6)(3-) and the proteolytic digestion of the gelatin film results an increase in the penetration of the Fe(CN)(6)(3-). Thus, by the change of peak potential in cyclic voltammogram of Fe(CN)(6)(3-), activity of the enzyme was determined.