Quantification of Moxifloxacin and Levofloxacin in Curettage Material by SPE-LC


NEMUTLU E., Katlan D., ÖZYÜNCÜ Ö., KIR S., BEKSAÇ M. S.

CHROMATOGRAPHIA, vol.71, pp.1147-1150, 2010 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 71
  • Publication Date: 2010
  • Doi Number: 10.1365/s10337-010-1596-8
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1147-1150

Abstract

Moxifloxacin and levofloxacin have been quantified in dilatation and curettage samples by reversed-phase LC. High recovery (> 91%) was obtained by simple and efficient solid-phase extraction. The method was validated in accordance with ICH guidelines to confirm specificity, linearity, accuracy, and precision. Response was a linear function of concentration over the range 0.010-30 mu g g(-1) with excellent correlation coefficients (> 0.997). Intra-day and inter-day precision and accuracy over the entire concentration range were less than 6.40 and 7.38%, respectively. Finally, the validated method was used for analysis of moxifloxacin and levofloxacin in dilatation and curettage material obtained from unwanted pregnancies up to 10 weeks gestation.