Quantification of Moxifloxacin and Levofloxacin in Curettage Material by SPE-LC

NEMUTLU E., Katlan D., ÖZYÜNCÜ Ö., KIR S., BEKSAÇ M. S.

CHROMATOGRAPHIA, cilt.71, ss.1147-1150, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 71
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1365/s10337-010-1596-8
  • Dergi Adı: CHROMATOGRAPHIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1147-1150
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Moxifloxacin and levofloxacin have been quantified in dilatation and curettage samples by reversed-phase LC. High recovery (> 91%) was obtained by simple and efficient solid-phase extraction. The method was validated in accordance with ICH guidelines to confirm specificity, linearity, accuracy, and precision. Response was a linear function of concentration over the range 0.010-30 mu g g(-1) with excellent correlation coefficients (> 0.997). Intra-day and inter-day precision and accuracy over the entire concentration range were less than 6.40 and 7.38%, respectively. Finally, the validated method was used for analysis of moxifloxacin and levofloxacin in dilatation and curettage material obtained from unwanted pregnancies up to 10 weeks gestation.