Time dependent change of ethanol consumption biomarkers, ethyl glucuronide and ethyl sulphate, after single dose ethanol intake

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Karacaoglu E., Odabasi A., AKÇAN R., TÜMER A. R., Lale A., KOCADAĞLI T., ...Daha Fazla

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, sa.3, ss.379-387, 2019 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1515/tjb-2018-0173
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.379-387
  • Anahtar Kelimeler: Ethyl glucuronide, Ethyl sulfate, Ethanol, Kinetics, Liquid chromatography-mass spectrometry, Polymorphism, UDP-GLUCURONOSYLTRANSFERASES, GENETIC POLYMORPHISMS, URINE, BLOOD, IDENTIFICATION, VOLUNTEERS, EXCRETION, PROFILES, SERUM
  • Hacettepe Üniversitesi Adresli: Evet

Özet

Background and objective: Ethyl glucuronide (EtG) and ethyl sulfate (EtS), minor metabolites of ethanol, aid to forensic scientist and clinicians to determine recent ethanol uptake when ethanol elimination is occurred. Present study aimed to show (a) kinetics of EtG and EtS in blood and urine after 0.5 g/kg ethanol intake (b) peak concentrations and time of disappearance in Turkish people. Material and methods: Seventeen (10 male, 7 female) healthy volunteers participated in the study. Blood and urine samples were obtained during 48 h after consumption and analyzed in LC-MS/MS. Results and conclusion: Blood peak concentrations of EtG and EtS were 0.13-0.389 mg/L and 0.211-0.5 mg/L, urine peak concentrations were 6.89-30.42 mg/L, and 10.5-58.17 mg/L, respectively. There was no EtG and EtS in all samples 24 h later in blood and 48 h later in urine. Correlation was found between EtG and EtS concentrations in blood. Most of obtained data are similar to literature, except EtS dominancy to EtG in urine and blood.