Metal-Chelating Nanopolymers for Antibody Purification from Human Plasma


AKTAŞ UYGUN D., Senay R. H. , Turkcan C., AKGÖL S., DENİZLİ A.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, cilt.168, sa.6, ss.1528-1539, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 168 Konu: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s12010-012-9875-5
  • Dergi Adı: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.1528-1539

Özet

The purification of immunoglobulin G (IgG) from human plasma was performed by using a novel metal-chelated adsorbent with nano size. The non-porous nanoparticles were produced by surfactant free emulsion polymerization of ethylene glycol dimethacrylate (EDMA) and 2-methacryloylamidohistidine (MAH). Then, Cu(II) ions were chelated on the nanoparticles. The nano-poly(EDMA-MAH) nanoparticles were characterized by Fourier transform infrared, scanning electron microscope, atomic force microscope and elemental analysis. The non-porous nanoparticles were spherical form and have 100-250 nm size distribution. The maximum IgG adsorption capacity of the Cu(II) chelated nanoparticles was found to be 463 mg/g polymer at pH 7.0 in HEPES buffer. Desorption of IgG was performed by 1.0 M NaCl and desorption rate was found to be 97 %. IgG was obtained from human plasma with purity of 94 % (up to 578 mg/g polymer). The non-porous nanoparticles allowed one-step purification of IgG from human plasma.