Immunohistochemical evaluation of periprosthetic membrane from loose cemented and uncemented total hip arthroplasty

Atilla B., Atilla P., TOKGÖZOĞLU A. M., Cakar A., Alpaslan A.

Saudi Medical Journal, vol.26, no.3, pp.429-433, 2005 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 3
  • Publication Date: 2005
  • Journal Name: Saudi Medical Journal
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.429-433
  • Hacettepe University Affiliated: Yes


Objective: Evaluate the cellular mechanisms responsible for the aseptic loosening of total hip replacements. Methods: Twenty periprosthetic membranes were collected during revision procedures at the Department of Orthopedics, Hacettepe University, Ankara, Turkey, during 1998 to 1999. Arthroplasties with polyethylene components and without polyethylene components were examined under light microscopy and immunohistochemically using the indirect immunoperoxidase technique into 2 different groups. Results: Immunohistochemical analysis with monoclonal antibodies reactive to certain cell surface antigens (CD45, CD44, CD98, CD31, CD26, CD71) revealed different morphological characteristics regarding the adjacent fibrosis, leukocyte activation, and immunologic response. Conclusion: These findings suggested the role of different cellular mechanisms in each group.