Partial purification of topoisomerase I from Mycobacterium phlei


PINAR A. , ACAN N. L. , Ozer N.

TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.38, ss.251-256, 2008 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 38 Konu: 3
  • Basım Tarihi: 2008
  • Dergi Adı: TURKISH JOURNAL OF MEDICAL SCIENCES
  • Sayfa Sayıları: ss.251-256

Özet

Aim: DNA topoisomerases control several cellular activities by catalyzing the relaxation of superhelices, which are produced during the replication of DNA. Investigation of the inhibitory action of the candidate substances on topoisomerase activity is widely used in drug development. The purpose of this study was to purify topoisomerase I from Mycobacterium phlei, which is closely related to Mycobacterium tuberculosis, and investigate some of its properties.