Preparation of magnetic nanoparticles-assisted plasmonic biosensors with metal affinity for interferon-alpha detection


Saylan Y., AKGÖNÜLLÜ S., DENİZLİ A.

MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, vol.280, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 280
  • Publication Date: 2022
  • Doi Number: 10.1016/j.mseb.2022.115687
  • Journal Name: MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Communication Abstracts, INSPEC, Metadex, Civil Engineering Abstracts
  • Keywords: Interferon-alpha detection, Magnetic nanoparticle, Plasmonic biosensor, Metal affinity, ULTRASENSITIVE DETECTION, CIBACRON BLUE, RESONANCE, PURIFICATION, IMMUNOSENSOR, CRYOGELS

Abstract

Sensitive, accurate, and rapid detection of biomolecules from complex matrices still remains a critical challenge. Herein, we designed magnetic nanoparticles which were coupled with plasmonic surfaces to detect a clinically important biomarker (interferon-alpha) in buffer solutions and artificial plasma samples. At first glance, the magnetic nanoparticles were synthesized and comprehensively characterized. Then, any potential external factors on magnetic nanoparticles were examined, thereby benchmarking detection performance. After determining optimum conditions, the plasmonic biosensor surface was modified with these magnetic nanoparticles for detecting interferon-alpha in real-time. The kinetic performance of magnetic nanoparticles-assisted plasmonic biosensor provided a high correlation coefficient (0.99) and a low limit of detection (41 IU/mL) in a broad range of interferon alpha concentrations. Consequently, this method holds a great potential to detect other biomolecules with high sensitivity and low interference and would be employed as an alternative approach for biomarker detection at different perspectives ranging from medical diagnosis to pharmaceutical industry.