Induction of apoptotic cell death in HL-60 cells by acteoside, a phenylpropanoid glycoside

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Inoue M., Sakuma Z., Ogihara Y., Saracoglu İ.

BIOLOGICAL & PHARMACEUTICAL BULLETIN, vol.21, no.1, pp.81-83, 1998 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 1
  • Publication Date: 1998
  • Doi Number: 10.1248/bpb.21.81
  • Journal Name: BIOLOGICAL & PHARMACEUTICAL BULLETIN
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.81-83
  • Keywords: acteoside, apoptosis, HL-60, phenylpropanoid glycoside, CONSTITUENTS, MECHANISM, AGENTS, H2O2, DNA
  • Hacettepe University Affiliated: Yes

Abstract

Acteoside, a phenylpropanoid glycoside with anti-oxidative activity, induced cell death in promyelocytic leukemia HL-60 cells with an IC50 value of 26.7 mu M. Analysis of extracted DNA on agarose gel electrophoresis revealed that acteoside induced the internucleosomal breakdown of chromatin DNA characteristic of apoptosis. Apoptosis-specific DNA fragmentation was clearly detectable 4 h after treatment with acteoside and was independent of the cell cycle phase. These data indicate that acteoside induces apoptosis in HL-60 cells.