Experimental Evaluation of the Effects of Ankaferd Blood Stopper and Collagenated Heterologous Bone Graft on Bone Healing in Sinus Floor Augmentation


Cakir M., Karaca I. R., FIRAT A., Kaymaz F., Bozkaya S.

INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS, vol.30, no.2, pp.279-285, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 30 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.11607/jomi.3793
  • Journal Name: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.279-285
  • Keywords: Ankaferd Blood Stopper, bone healing, collagenated heterologous bone graft, sinus augmentation, tartrate-resistant acid phosphatase, PORCINE BONE, POSTERIOR MAXILLA, ENDOSSEOUS IMPLANTS, ELEVATION, SUBSTITUTE, PARTICLES, TISSUE, REGENERATION, EXPRESSION, RABBITS
  • Hacettepe University Affiliated: Yes

Abstract

Purpose: The aim of this study was to evaluate the effect of collagenated heterologous bone graft (CHBG) and Ankaferd Blood Stopper (ABS), a plant extract, on bone healing after sinus floor augmentation. Materials and Methods: Thirty-six New Zealand rabbits were used. Bilateral sinus augmentation was performed, and 72 bone defects were created. The maxillary sinuses were grafted with four different biomaterials: blood clot (control group), CHBG (Apatos Mix, OsteoBiol, Tecnoss) (graft group), ABS (ABS group), and ABS + CHBG (ABS+graft group). The rabbits were sacrificed at 1, 4, and 8 weeks after surgery. Histochemical and immunohistochemical examinations were performed on all samples. Staining with hematoxylin-eosin and Masson trichrome was performed, and bone marker activity was evaluated. Results: Lymphocyte infiltration was high at the first week in all groups and decreased from 1 to 8 weeks. All materials were biocompatible. Osteoclast numbers increased in the control group from 1 to 8 weeks and decreased in the other groups. There was no new bone formation in week 1 in all groups. New bone formation increased in all groups from 1 to 8 weeks, and at the fourth week, new bone formation was greater in the ABS and ABS+graft groups than in the other groups. There were osteoclasts around the bone graft materials, but degeneration of the graft was seen only in the ABS+graft group at week 8. Conclusion: ABS accelerates bone healing in sinus augmentation procedures and can be used alone or with CHBG. CHBG has osteoconductive properties, and ABS can accelerate bone graft degeneration.