The effect of salt stress (50, 100 and 150 nM NaCl) on the endogenous levels of free, bound and total polyamines was studied in root tissues of salt tolerant (Coban) and salt sensitive (Sanbro) cultivars of sunflower (Helianthus ammuus L.) plants for the 5(th), 15(th) and 25(th) days of the growth periods. The amounts of free, AS-bound and total Spm increased in root tissues of sunflower plants subjected to salt stress for different periods of time while the levels, of other polyamine titers were Or unchanged significantly. The increase in some polyamine titers in either decreased in general or unchanged significantly. The increase in some polyamine titer in sunflower root tissues under salinization suggests their possible role in combating the adverse effect of salinity stress. The salt tolerance in sunflower plants was related to the excessive accumulation of total polyamines in root tissues of salt tolerant cultivar Coban under saline condition.