Influence of in utero di-n-hexyl phthalate and dicyclohexyl phthalate on fetal testicular development in rats

Ahbab M. A., BARLAS N.

TOXICOLOGY LETTERS, cilt.233, sa.2, ss.125-137, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 233 Sayı: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.toxlet.2015.01.015
  • Dergi Adı: TOXICOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.125-137
  • Anahtar Kelimeler: Di-n-hexyl phthalate, Dicyclohexyl phthalate, Endocrine disrupters, Aantiandrogen, Testicular development, In utero, DOSE-DEPENDENT ALTERATIONS, REPRODUCTIVE-TRACT, ANDROGEN-RECEPTOR, LACTATIONAL EXPOSURE, LEYDIG-CELLS, TESTOSTERONE PRODUCTION, SEXUAL-DIFFERENTIATION, DI(N-BUTYL) PHTHALATE, FUNCTIONAL MATURATION, ANOGENITAL DISTANCE
  • Hacettepe Üniversitesi Adresli: Evet

Özet

This study investigated the effects of di-n-hexyl phthalate (DHP) and dicyclohexyl phthalate (DCHP) on male reproductive development in utero. Pregnant rats were exposed to DHP and DCHP at doses of 0 (vehicle), 20, 100 and 500 mg/kg/day, by gavage, on gestational days (GD) 6-19. A significant decrease in the anogenital distance (AGD) of male fetuses was observed at all doses of DHP and DCHP. The AGD/cube root of body weight ratio in male fetuses was also significantly reduced compared to control group. The litters with resorption, percentage of resorptions and inhibin B levels increased in treatment groups. Moreover, testosterone and MIS/AMH levels in all treatment groups decreased. Although FSH and inhibin B levels of male pups exposed to DHP and DCHP increased, FSH/inhibin B ratio decreased in treatment groups. Reduced testosterone production in response to DHP and DCHP exposure appeared to be related to changes in testosterone metabolism, as shown by decreased 3 beta-HSD immunoexpression. The percentages of large Leydig clusters increased after exposure to DHP and DCHP in utero. Histopathological examination of the testis on GD20 revealed changes at all doses. Relative integrated immunodensities of 3 beta-HSD, MIS/AMH, PCNA and AR decreased after DHP and DCHP exposures. Altered fetal Sertoli cell development and function may be caused by disrupted PMC function revealed by reduced AR production in these cells in treatment groups. (C) 2015 Elsevier Ireland Ltd. All rights reserved.