Utility of a commercial quantitative hepatitis C virus core antigen assay in a diagnostic laboratory setting

ERGÜNAY K., ŞENER B., ALP A., Karakaya J., Hascelik G.

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol.70, no.4, pp.486-491, 2011 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 70 Issue: 4
  • Publication Date: 2011
  • Doi Number: 10.1016/j.diagmicrobio.2011.04.011
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.486-491


In this study, the utility and impact of hepatitis C virus (HCV) core antigen (Cag) detection via a commercial assay have been evaluated in diagnostic laboratory conditions. In a total of 272 samples from 226 individuals, HCV RNA was detected in 81.3% and anti-HCV antibody prevalence was 86.4%. HCV Cag reactivity was identified in 59.9% of the samples and in 75.8% with detectable RNA. The sensitivity and specificity of HCV Cag assay have been calculated as 75.8% and 95.1%, respectively, and agreement between HCV RNA and HCV Cag was moderate (kappa = 0.554). HCV Cag and RNA levels were highly correlated (r = 0.915 and 0.937). A viral load threshold of 10(3) IU/mL has been recognized, above which the correlation with RNA became statistically significant and sensitivity increased to 90.9%. Detection and quantification of HCV core antigen have been observed as a strong alternative to nucleic acid testing for HCV monitorization. (C) 2011 Elsevier Inc. All rights reserved.