Simultaneous Quantification of Paclitaxel and Vinorelbine Encapsulated in Theranostic Nanosized Liposomes

KARPUZ M., DOĞAN A., NEMUTLU E., SİLİNDİR GÜNAY M., Ozer A. Y.

JOURNAL OF ANALYTICAL CHEMISTRY, vol.76, no.6, pp.742-748, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 76 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1134/s1061934821060034
  • Journal Name: JOURNAL OF ANALYTICAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Compendex, Food Science & Technology Abstracts
  • Page Numbers: pp.742-748
  • Keywords: paclitaxel, vinorelbine, liposome, HPLC, determination, PHARMACEUTICAL ANALYSIS, VALIDATION
  • Hacettepe University Affiliated: Yes

Abstract

Cancer is one of the major health problems with an increasing incidence and mortality. Although imaging and effective treatment methods are critical issues to prevent cancer mortality, they are inadequate. Therefore, new approaches including combined treatment protocol and nanosized drug delivery theranostic systems are being investigated because of the deficiency of conventional cancer imaging and treatment methods in clinics. Liposomes attract attention in cancer imaging and treatment due to their advantages. In this study, an HPLC method was developed for simultaneous quantification of paclitaxel (PCX) and vinorelbine tartarate (VNB) in co-drug encapsulated theranostic nanosized liposome formulations as an effective cancer imaging and treatment agent. The method was validated according to ICH Analytical Method Validation guideline. According to the validation results, the method was found to be linear (r > 0.999), accurate (bias <= 1.8), precise (RSD <= 1.8%) and robust (p > 0.05). The developed method was successfully applied to the quantification of PCX and VBN in liposomes for encapsulation efficiency.