Dye-attached magnetic poly(hydroxyethyl methacrylate) nanospheres for albumin depletion from human plasma
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.43, sa.1, ss.62-70, 2015 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 43 Sayı: 1
- Basım Tarihi: 2015
- Doi Numarası: 10.3109/21691401.2013.841172
- Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.62-70
- Anahtar Kelimeler: albumin depletion, dye-ligand affinity, magnetic nanospheres, proteomics, PHEMA, HUMAN SERUM, PROTEOMIC ANALYSIS, LYSOZYME PURIFICATION, EFFICIENT REMOVAL, AFFINITY, PROTEINS, NANOPARTICLES, BINDING, BEADS, COMBINATION
- Hacettepe Üniversitesi Adresli: Evet
Özet
The selective binding of albumin on dye-affinity nanospheres was combined with magnetic properties as an alternative approach for albumin depletion from human plasma. Magnetic poly(hydroxyethyl methacrylate) (mPHEMA) nanospheres were synthesized using mini-emulsion polymerization method in the presence of magnetite powder. The specific surface area of the mPHEMA nanospheres was found to be 1302 m(2)/g. Subsequent to Cibacron Blue F3GA (CB) immobilization onto mPHEMA nanospheres, a serial characterization processing was implemented. The quantity of immobilized CB was calculated as 800 mu mol/g. Ultimately, albumin adsorption performance of the CB-attached mPHEMA nanospheres from both aqueous dissolving medium and human plasma were explored.