Dye-attached magnetic poly(hydroxyethyl methacrylate) nanospheres for albumin depletion from human plasma

GOKAY O., KARAKOC V., Andac M., TÜRKMEN D., DENİZLİ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.43, no.1, pp.62-70, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 43 Issue: 1
  • Publication Date: 2015
  • Doi Number: 10.3109/21691401.2013.841172
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.62-70
  • Keywords: albumin depletion, dye-ligand affinity, magnetic nanospheres, proteomics, PHEMA, HUMAN SERUM, PROTEOMIC ANALYSIS, LYSOZYME PURIFICATION, EFFICIENT REMOVAL, AFFINITY, PROTEINS, NANOPARTICLES, BINDING, BEADS, COMBINATION
  • Hacettepe University Affiliated: Yes

Abstract

The selective binding of albumin on dye-affinity nanospheres was combined with magnetic properties as an alternative approach for albumin depletion from human plasma. Magnetic poly(hydroxyethyl methacrylate) (mPHEMA) nanospheres were synthesized using mini-emulsion polymerization method in the presence of magnetite powder. The specific surface area of the mPHEMA nanospheres was found to be 1302 m(2)/g. Subsequent to Cibacron Blue F3GA (CB) immobilization onto mPHEMA nanospheres, a serial characterization processing was implemented. The quantity of immobilized CB was calculated as 800 mu mol/g. Ultimately, albumin adsorption performance of the CB-attached mPHEMA nanospheres from both aqueous dissolving medium and human plasma were explored.