Akademik Veri Yönetim Sistemi
XXXI. Türk Biyokimya Derneği Ulusal Biyokimya Kongresi 2020, 18 - 20 Aralık 2020, ss.79
OBJECTIVES: 592 of the 727 samples sent to Hacettepe University Hospitals
Forensic Toxicology Laboratory between 01/01/2016 and 31/12/2019 in order
to confirm positive substances by AMATEM (Alcohol and Substance Addiction
Treatment and Training Center) with immunoassay method were detected as
positive. It is known that additional substances and drugs are also abused in people
who abuse any substance, which are not included in the routine immunoassay
screening panel. In addition to routine screening and subsequent confirmation,
urine samples were subjected to a broad screening by GC-MS (gas chromatographymass spectrometry) in order to investigate the presence of other abused substances.
MATERIALS and METHODS: 98 of the urine samples that came to our
laboratory in 2018 with a confirmation request and were stored at -20°C
after analysis were randomly selected and re-analyzed using the GC-MS
method. Liquid-liquid and SPE extraction was applied to the samples.
RESULTS: Between 01/01/2018 and 31/12/2018, 301 samples were sent
to our laboratory for confirmation, 237 of which were found positive.
Additional substances such as Pregabalin, Gabapentin, Codeine, Naproxen,
Pseudoephedrine, Ephedrine, Ibuprofen, Caffeine, Tributylamine, Theobromine,
Cotinine, and JWH-073 were detected in the re-analyzed samples.
CONCLUSIONS: The substances detected in the immunoassay screening
method used in AMATEMs are known by those who benefit from the probation
facility and these people especially avoid these substances. As shown in our
study, people under probation frequently use alternative substances. For this reason, questioning and including such substances in screening beside classical
drugs will provide important information in the follow-up of drug addicts.
Keywords: Confirmation, GC-MS, Multiple Substance Abuse, Substance
Screening