Purpose: To investigate the effect of recombinant follicular stimulating hormone (r-FSH) and human menopausal gonadotropin (hMG) on follicular microenvironment via assessment of follicular and serum vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1) levels in intracytoplasmic sperm injection (ICSI) cycles. Materials and Methods: Designed as a prospective cohort study. Twenty-five patients underwent controlled ovarian hyperstimulation (COH) with r-FSH and 20 patients underwent with hMG. Results: Both groups were comparable regarding the women's mean age and body mass index (BMI). The amount of VEGF (pg/ml) in serum and follicular fluid in the group I and II were comparable (275 +/- 135.3 vs 330.7 +/- 190.0; p > 0.05 and 2,081.1 +/- 1095.1 vs 1,971.1 +/- 975.6; p > 0.05, respectively). The amount of IGF-1 (ng/ml) in serum and follicular fluid in the group I and II were also comparable (225.3 +/- 69.3 vs 204.1 +/- 56.3, p > 0.05 and 176.1 +/- 67.2 vs 185.8 +/- 48.7,p > 0.05, respectively). Pregnancy rates were also comparable between groups. Conclusions: The hMG and r-FSH in COH produced comparable follicular microenvironment regarding follicular VEGF and IGF-1.