Electrochemical determination of beta-lactoglobulin in whey proteins

Surucu O., ABACI S.

JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, vol.14, no.1, pp.11-19, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.1007/s11694-019-00262-w
  • Journal Name: JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, CAB Abstracts, Compendex, Food Science & Technology Abstracts, Veterinary Science Database
  • Page Numbers: pp.11-19
  • Hacettepe University Affiliated: Yes

Abstract

Determination of beta-lactoglobulin in milk products is very important because beta-lactoglobulin is the main ingredient in whey-based protein powders. However, beta-lactoglobulin is a dangerous food allergen. On such an occasion, the determination of beta-lactoglobulin is coming into prominence and electrochemistry is a good alternative for this purpose because of its simple, economic and rapid response. In this work, a graphene oxide modified pencil graphite electrode is developed to determine beta-lactoglobulin based on the current signal of known concentration of hydrogen peroxide. Cyclic voltammetry technique is performed to obtain the electrochemical behavior of beta-lactoglobulin. Linear range, limit of detection and limit of quantification were calculated according to the calibration curve of various amounts of beta-lactoglobulin. Ultraviolet-visible spectroscopy technique is also used to investigate the absorption behavior of beta-lactoglobulin in various biological macromolecules including whey proteins. The proposed graphene oxide modified pencil graphite surface is successfully applied to determine beta-lactoglobulin in real milk sample, so a new methodology based on a newly developed electrochemical technique is described as a promising alternative in diary products.