A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

ÇELEBİER, MUSTAFA; Ibanez, Clara; Simo, Carolina; Cifuentes, Alejandro

doi:10.1007/978-1-4939-8793-1_26

A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

ÇELEBİER M., Ibanez C., Simo C., Cifuentes A.

ELECTROPHORETIC SEPARATION OF PROTEINS, ss.303-313, 2019 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Basım Tarihi: 2019
Doi Numarası: 10.1007/978-1-4939-8793-1_26
Dergi Adı: ELECTROPHORETIC SEPARATION OF PROTEINS
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.303-313
Hacettepe Üniversitesi Adresli: Evet

Özet

The potential of capillary electrophoresis-mass spectrometry (CE-MS) for metabolomics is demonstrated through the analysis of metabolites from human HT29 colon cancer cells treated and non-treated with dietary polyphenols. Prior to CE-MS analysis, four different metabolite purification strategies are investigated. Namely, the results obtained after methanol deproteinization, ultrafiltration, and two solid-phase extraction methods using C18 and polymer-based cartridges are described. These generic methods can have broad applications to analyze metabolites in a large variety of matrices and fields, including the new Foodomics area.