Although burnout has been researched widely with regard to nurses working in different settings, until now it has not been investigated among stem cell transplantation unit nurses. This study's aim was to determine the burnout level of 57 nurses who were working in stem cell transplantation units in Turkey. The research instruments that were used included a form of demographic characteristics and the Maslach Burnout Inventory. For all the hematopoietic stem cell transplantation nurses, the mean subscale scores for emotional exhaustion were the highest, followed by personal accomplishment and then depersonalization. The burnout level rose with increasing age and duration on the job. Interventions to reduce burnout are needed at both the administrative and organizational levels. In addition, it is essential to address and prevent the problems that are related to burnout, especially among nurses who work in the same unit for a long time.