In vitro anticholinesterase activity and molecular docking studies of coumarin derivatives isolated from roots of Heptaptera cilicica


ÖZBEK H., GÜVENALP Z., Yilmaz G., Yerdelen K. O. , KAZAZ C., Demirezer O. L.

MEDICINAL CHEMISTRY RESEARCH, vol.27, no.2, pp.538-545, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.1007/s00044-017-2080-x
  • Title of Journal : MEDICINAL CHEMISTRY RESEARCH
  • Page Numbers: pp.538-545
  • Keywords: Heptaptera cilicica, Apiaceae, Coumarin, AChE, BuChE, L.

Abstract

The chloroform extract of the roots of Heptaptera cilicica (Boiss. & Bal.) Tutin (Apiaceae) was investigated in terms of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory effects by Ellman method. Afterwards, a new furocoumarin: trichoclin angelate with five known coumarin derivatives: umbelliprenin, badrakemone, badrakemin, badrakemin acetate and prunate were isolated from this extract. Their structures were identified by means of spectroscopic methods (1D, 2D-NMR and HRESIMS). The next step of our study was determining AChE and BuChE inhibitory activities of the compounds by molecular docking and in vitro methods. According to the results, prunate was found to be the most potent compound, which exhibited significant inhibitory potency against acetylcholinesterase (IC50 = 1.76 +/- 0.003 A mu M) and butyrylcholinesterase (IC50 = 0.21 +/- 0.002 mu M) as compared with the reference compound, galantamine hydrobromide.