In vitro anticholinesterase activity and molecular docking studies of coumarin derivatives isolated from roots of Heptaptera cilicica

ÖZBEK H., GÜVENALP Z., Yilmaz G., Yerdelen K. O., KAZAZ C., Demirezer O. L.

MEDICINAL CHEMISTRY RESEARCH, cilt.27, sa.2, ss.538-545, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 27 Sayı: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s00044-017-2080-x
  • Dergi Adı: MEDICINAL CHEMISTRY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.538-545
  • Anahtar Kelimeler: Heptaptera cilicica, Apiaceae, Coumarin, AChE, BuChE, L.
  • Hacettepe Üniversitesi Adresli: Evet

Özet

The chloroform extract of the roots of Heptaptera cilicica (Boiss. & Bal.) Tutin (Apiaceae) was investigated in terms of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory effects by Ellman method. Afterwards, a new furocoumarin: trichoclin angelate with five known coumarin derivatives: umbelliprenin, badrakemone, badrakemin, badrakemin acetate and prunate were isolated from this extract. Their structures were identified by means of spectroscopic methods (1D, 2D-NMR and HRESIMS). The next step of our study was determining AChE and BuChE inhibitory activities of the compounds by molecular docking and in vitro methods. According to the results, prunate was found to be the most potent compound, which exhibited significant inhibitory potency against acetylcholinesterase (IC50 = 1.76 +/- 0.003 A mu M) and butyrylcholinesterase (IC50 = 0.21 +/- 0.002 mu M) as compared with the reference compound, galantamine hydrobromide.