"Volatile Anesthetics and Their Association with Oxidative Stress"


Sayal A., Kartal Y., Kayaaltı Z., Aydın A., Türkan H., Karahalil B.

8th Turkish Society of Toxicology Congress, Antalya, Turkey, 15 - 18 November 2012, pp.38

  • Publication Type: Conference Paper / Summary Text
  • City: Antalya
  • Country: Turkey
  • Page Numbers: pp.38

Abstract

General anaesthetics are frequently used in patients with under oxidative stress by the reason of not only a serious illness but also surgical trauma. Some of anaesthetics may worsen oxidative stress on the other hand some of them may act as antioxidants. Sevoflurane and desflurane volatile anesthetics are used commonly for induction and maintance of general anesthesia in anesthetic practice. In our first study, we aimed to observe tissue response of rat that was exposed to sevoflurane and desflurane by analyzing the oxidative stress in liver, brain, kidney and lung tissues, All tissues were analyzed using parameters of oxidative stress, malondialdehyde (MDA) with TBARS test, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and levels of Cu and Zn. In the first study, animals divided into three groups as control group, sevoflurane group and desflurane group. This study showed that exposure to sevoflurane and desflurane caused oxidative stress which was shown by increased concentration of MDA in lung tissue much more affected from ROS than other tissues due to first tissue exposed to anesthetic gases. While the levels of MDA were statistically significantly decreased in liver in desflurane group but increased in sevoflurane group. It suggested that desflurane were more protective effect than sevoflurane due to decreased MDA levels in liver and there is a statistical significant relationship between sevoflurane group and desflurane group compared to control group in terms of oxidative status. In our second study, we purposed to compare the markers of oxidative status of human erythrocyte in both sevoflurane and desflurane. In order to this, venous blood samples of patients who scheduled for abdominal surgery were collected the following time intervals; initial time and first hour, first day and third days after anesthesia (sevoflurane and desflurane). In addition, the levels of MDA, GSH-Px, SOD, Mg and Zn were determined. No significant diffirences were observed in these measurements at 1st hour, 1st and 3st days compared to initial time in desflurane group (p>0.05). But in the sevoflurane group, statistically significant differences were found. In a conclusion of our studies, we investigated tissue oxidative stress and enzymatic antioxidant activity of desflurane and sevoflurane anesthetic agents (p<0.05). The authors suggest that further studies with other nonenzymatic indices of oxidative stress such as tocopherols, ascorbate, and 8OHdG can help enlighting this anesthetic agent selection.