Research Information System
8th Turkish Society of Toxicology Congress, Antalya, Turkey, 15 - 18 November 2012, pp.38
General anaesthetics are frequently used in patients
with under oxidative stress by the reason of not only a serious illness but
also surgical trauma. Some of anaesthetics may worsen oxidative stress on the
other hand some of them may act as antioxidants. Sevoflurane and desflurane
volatile anesthetics are used commonly for induction and maintance of general
anesthesia in anesthetic practice. In our first study, we aimed to observe tissue
response of rat that was exposed to sevoflurane and desflurane by analyzing the
oxidative stress in liver, brain, kidney and lung tissues, All tissues were
analyzed using parameters of oxidative stress, malondialdehyde (MDA) with TBARS
test, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and levels of
Cu and Zn. In the first study, animals divided into three groups as control
group, sevoflurane group and desflurane group. This study showed that exposure
to sevoflurane and desflurane caused oxidative stress which was shown by
increased concentration of MDA in lung tissue much more affected from ROS than
other tissues due to first tissue exposed to anesthetic gases. While the levels
of MDA were statistically significantly decreased in liver in desflurane group
but increased in sevoflurane group. It suggested that desflurane were more
protective effect than sevoflurane due to decreased MDA levels in liver and
there is a statistical significant relationship between sevoflurane group and
desflurane group compared to control group in terms of oxidative
status. In our second study, we purposed to compare the markers of oxidative
status of human erythrocyte in both sevoflurane and desflurane. In order to
this, venous blood samples of patients who scheduled for abdominal surgery were
collected the following time intervals; initial time and first hour, first day
and third days after anesthesia (sevoflurane and desflurane). In addition, the
levels of MDA, GSH-Px, SOD, Mg and Zn were determined. No significant diffirences
were observed in these measurements at 1st hour, 1st and 3st days compared to
initial time in desflurane group (p>0.05). But in the sevoflurane group, statistically
significant differences were found. In a conclusion of our studies, we
investigated tissue oxidative stress and enzymatic antioxidant activity of desflurane
and sevoflurane anesthetic agents (p<0.05). The authors suggest that further
studies with other nonenzymatic indices of oxidative stress such as
tocopherols, ascorbate, and 8OHdG can help enlighting this anesthetic agent
selection.