Construction of a Metagenomic Library for the Marine Sponge Halichondria okadai
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, cilt.76, sa.4, ss.633-639, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 76 Sayı: 4
- Basım Tarihi: 2012
- Doi Numarası: 10.1271/bbb.110533
- Dergi Adı: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.633-639
- Hacettepe Üniversitesi Adresli: Evet
Özet
Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454-pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alpha-proteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria., and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.