The aim of this study is the development of novel cell support materials for fibroblast cell cultivation by using low-pressure plasma assisted treatment. Poly(ε-caprolactone) (PCL) membranes were prepared by solvent-casting technique. The plasma assisted treatment was focused on generating a nano-topography and obtaining COOH functionalities on the surface of the membranes. The immobilization of biomolecules onto the PCL membranes was realized after the plasma treatment. The membranes prepared were characterized by various methods before and after the bio-modification. L929 mouse fibroblasts were used for cell culture evaluation. The prominent roles of surface nano-topography and carboxylic groups generated by the plasma in obtaining better cell growth on PCL surfaces are highlighted in this study. © 2009 Springer Science + Business Media B.V.