Chiral separation-based ligand exchange by open-tubular capillary electrochromatography
ANALYTICAL BIOCHEMISTRY, cilt.447, ss.55-57, 2014 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 447
- Basım Tarihi: 2014
- Doi Numarası: 10.1016/j.ab.2013.11.006
- Dergi Adı: ANALYTICAL BIOCHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.55-57
- Hacettepe Üniversitesi Adresli: Evet
Özet
Chiral ligand-exchange enantioseparation of aliphatic and aromatic amino acids was successfully performed using a new open-tubular zwitterionic column with tentacle-type polymer stationary phase. The polymeric stationary phase was prepared using 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl), a new reactive monomer. The preparation procedure of the open-tubular column included silanization, in situ graft polymerization with HPMA-Cl, and L-histidine (L-His) modification. L-His was used as a chiral ligand-exchange selector and copper(II) as a central ion. Successful enantioseparation of six pairs of amino acid enantiomers was achieved with a buffer of 5 mM CuSO4, 20 mM (NH4)(2)SO4 at pH 3.0. (C) 2013 Elsevier Inc. All rights reserved.