Chiral separation-based ligand exchange by open-tubular capillary electrochromatography

Aydogan C., DENİZLİ A.

ANALYTICAL BIOCHEMISTRY, vol.447, pp.55-57, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 447
  • Publication Date: 2014
  • Doi Number: 10.1016/j.ab.2013.11.006
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.55-57
  • Hacettepe University Affiliated: Yes


Chiral ligand-exchange enantioseparation of aliphatic and aromatic amino acids was successfully performed using a new open-tubular zwitterionic column with tentacle-type polymer stationary phase. The polymeric stationary phase was prepared using 3-chloro-2-hydroxypropyl methacrylate (HPMA-Cl), a new reactive monomer. The preparation procedure of the open-tubular column included silanization, in situ graft polymerization with HPMA-Cl, and L-histidine (L-His) modification. L-His was used as a chiral ligand-exchange selector and copper(II) as a central ion. Successful enantioseparation of six pairs of amino acid enantiomers was achieved with a buffer of 5 mM CuSO4, 20 mM (NH4)(2)SO4 at pH 3.0. (C) 2013 Elsevier Inc. All rights reserved.