Acute long-distance trail running increases serum IL-6, IL-15, and Hsp72 levels

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Yargic M. P., Torgutalp S., AKIN Ş., BABAYEVA N., Torgutalp M., Demirel H. A.

APPLIED PHYSIOLOGY NUTRITION AND METABOLISM, vol.44, no.6, pp.627-631, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 6
  • Publication Date: 2019
  • Doi Number: 10.1139/apnm-2018-0520
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.627-631
  • Keywords: running, endurance training, exercise metabolism, exercise endocrinology, cytokines, stress, HEAT-SHOCK PROTEINS, SKELETAL-MUSCLE, EXERCISE, INTERLEUKIN-6, RELEASE, HEAT-SHOCK-PROTEIN-72, PERFORMANCE, CELLS, SECRETION, CYTOKINES
  • Hacettepe University Affiliated: Yes


Interleukin-6 (IL-6), IL-15, and heat shock protein 72 (Hsp72) are molecules that have significant metabolic effects on glucose and fat metabolism and a cell's stress response. The aim of this study is to determine serum levels of these molecules in runners after a long-distance trail run. Serum IL-15 levels after such endurance events have not been investigated yet. Blood samples were collected from 37 athletes (11 female, 26 male) before and after a 35-km trail run, with a total climb of 940 m. Serum was obtained from the samples, and IL-6, IL-15, and Hsp72 levels were measured from using the sandwich ELISA method. The athletes completed the race in 308.3 +/- 37.4 min on average. After the race, the mean serum IL-6, IL-15, and Hsp72 concentrations increased 13.2-fold, 2.22-fold, and 1.6-fold, respectively (p < 0.001, p < 0.001, and p = 0.039, respectively). This is the first study to demonstrate the increase in serum IL-15 levels following an acute endurance exercise. In addition to IL-15, we report that IL-6 and soluble Hsp72 levels also increased significantly following a 35-km trail run. Since these molecules are involved in regulating glucose and fat metabolism, significant increases of IL-6, IL-15, and soluble Hsp72 may have health benefits that may be associated with long-distance trail runs, which are becoming more popular worldwide.