2nd Zebrafish Workshop, Turkey, Ankara, Turkey, 18 March 2021, pp.7
Zebrafish is an attractive vertebrate model organism for rare disease research. Introduction of point mutations in the zebrafish genome would be necessary to model diseases however this has been still a challenge in zebrafish. As a part of our project‘’Creating Rare Disease Specific CRISPR-Cas9 Platforms in Zebrafish and Ensuring Their Sustainability’’, we aimed to introduce a target missense mutation in zebrafish genome and optimize our protocol for this work package. Our target gene for this aim is tgfbi. Despite using nine different injection protocols including standard ones and novel different combinations from literature we could not achieve knock-in of the precise mutation in the target sequence Therefore, introduction of precise missense mutations to zebrafish genome is still a challenge in our laboratory as it is the case for many other zebrafish researchers. However, we will continue to try and optimize protocols via using novel methods such as base editing and prime editing. This project was funded Hacettepe University (Grant reference:TAY-2017-12735).