Quartz crystal microbalance based histidine sensor
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.47, sa.1, ss.221-227, 2019 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 47 Sayı: 1
- Basım Tarihi: 2019
- Doi Numarası: 10.1080/21691401.2018.1548474
- Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.221-227
- Hacettepe Üniversitesi Adresli: Evet
Özet
Herein, quartz crystal microbalance (QCM) biosensor is prepared for the detection of L-histidine by attachment of L-histidine imprinted poly(EGDMA-MAH/Cu(II)) nanoparticles on QCM electrode. The imprinted nanoparticles with the size of 86.43 nm were synthesized via miniemulsion polymerization reaction. Prepared QCM sensors were characterized with ellipsometer, contact angle measurements and FTIR. The thickness measurements demonstrated that the particle thin films were almost mono-layer. L-histidine solutions with a concentration range between 6.44 mu M and 225.6 mu M were introduced to QCM system to determine the adsorption kinetics. Selectivity of the L-histidine imprinted nanoparticles were examined using D-histidine and L-tryptophan as competitor molecules. L-histidine imprinted QCM biosensors was also used for RNAase, lysozyme, cytochrome-C and BSA to investigate the competitive adsorption of surface histidine exposed proteins.