Optimization and physicochemical characterization of enhanced microbial cellulose production with a new Kombucha consortium

AVCIOĞLU N. H., Birben M., SEYİS BİLKAY I.

Process Biochemistry, cilt.108, ss.60-68, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 108
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.procbio.2021.06.005
  • Dergi Adı: Process Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.60-68
  • Anahtar Kelimeler: Microbial cellulose, Kombucha culture, FIB-SEM, FT-IR, TGA, XRD, BACTERIAL CELLULOSE, KOMAGATAEIBACTER-RHAETICUS, BY-PRODUCT, GLUCONACETOBACTER-XYLINUS, INDUSTRIAL-WASTE, NITROGEN-SOURCES, CARBON-SOURCES, STRAIN, TEA, FERMENTATION
  • Hacettepe Üniversitesi Adresli: Evet

Özet

© 2021 Elsevier LtdBacterial cellulose (BC) is a natural biopolymer, used in biomedical, food, paper, electronics and other industrial processes. The objective of this research was to improve the productivity of cellulose by using a new kombucha consortium (Komagataeibacter saccharivorans LN886705, Brettanomyces bruxellensis MH393498 and Brettanomyces anomalus KY103303) with a low-cost nitrogen source (black tea). Accordingly, BC can be highly produced (18.68 g/L BC) in optimized media (containing 1% black tea and 6% glucose, pH 6) at 30 °C, for 10 days under static cultivation. Results demonstrated that cellulose production enhanced 4.06 times in optimized medium when compared with Hestrin-Schramm medium (4.59 g/L BC) with a 29.74% cost reduction. Characterization of obtained cellulose was performed by using Focussed Ion Beam Scanning Electron Microscopy (FIB-SEM),Fouirer Transform Infrared (FT-IR) Spectrometer, Thermogravimetric Analysis (TGA-DSC) and X-Ray Diffraction (XRD). Cellulose fibrils showed a typical porous, cellulose type I structure and exhibits high thermal stability with a total weight loss of 76%. Results confirmed that, it was possible to obtain high value cellulose with excellent physicochemical properties and cost-reduction by using low-cost nitrogen source.