Prolonged retention and in vivo evaluation of cationic nanoparticles loaded with Mitomycin C designed for intravesical chemotherapy of bladder tumours

Erdogar N., İSKİT A. B., Mungan N. A., BİLENSOY E.

Journal of Microencapsulation, cilt.29, sa.6, ss.576-582, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.3109/02652048.2012.668957
  • Dergi Adı: Journal of Microencapsulation
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.576-582
  • Anahtar Kelimeler: bladder cancer, bladder retention, cationic nanoparticles, chitosan, intravesical drug delivery, poly-epsilon-caprolactone, poly-L-lysine, rat model, CHITOSAN NANOPARTICLES, DRUG-DELIVERY, CANCER, PERMEABILITY, NANOSPHERES, CHALLENGES, STABILITY, WALL
  • Hacettepe Üniversitesi Adresli: Evet

Özet

To overcome the recurrence problem in bladder tumours; nanoparticles with positive surface charge may improve interaction with biological membranes for intravesical administration. The aim of this study was to design, develop and evaluate (in vitroin vivo) cationic nanoparticles based on chitosan, poly-L-lysine or polycaprolactone for the effective intravesical delivery of chemotherapeutic agent MMC in a rat model. Poly-L-lysine-coated polycaprolactone nanoparticles and chitosan-coated polycaprolactone nanoparticles were prepared by the double emulsion technique. Chitosan nanoparticles were prepared by ionic gelation. It was found that nanoparticle formulations of 160320nm in size can be produced in 1435 encapsulation efficiency. Variability in the particle size of nanoparticles depended on the preparation method. Encapsulation was increased by two-fold for CSPCL as a result of the double emulsion technique. Commercial MMC product in solution form and cationic nanoparticle formulations were compared for in vivo bladder retention properties and effect of formulations on urine volume. © 2012 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.