Global Phylogenomic Analysis of Nonencapsulated Streptococcus pneumoniae Reveals a Deep-Branching Classic Lineage That Is Distinct from Multiple Sporadic Lineages

Hilty, ÖZGEN; Wuethrich, Daniel; Salter, Susannah; Engel, Hansjuerg; Campbell, Samuel; Sa-Leao, Raquel; De Lencastre, Herminia; Hermans, Peter; Sadowy, Ewa; Turner, Paul; Chewapreecha, Claire; Diggle, Mathew; Pluschke, Gerd; Mcgee, Lesley; Eser, Ozgen; Low, Donald; Smith-Vaughan, Heidi; Endimiani, Andrea; Kueffer, Marianne; Dupasquier, Melanie; Beaudoing, Emmanuel; Weber, Johann; Bruggmann, Remy; Hanage, William; Parkhıll, Julian; Hathaway, Lucy; Muehlemann, Kathrin; Bentley, Stephen

doi:10.1093/gbe/evu263

Global Phylogenomic Analysis of Nonencapsulated Streptococcus pneumoniae Reveals a Deep-Branching Classic Lineage That Is Distinct from Multiple Sporadic Lineages

Atıf İçin Kopyala

Hilty M., Wuethrich D., Salter S. J., Engel H., Campbell S., Sa-Leao R., ...Daha Fazla

GENOME BIOLOGY AND EVOLUTION, cilt.6, ss.3281-3294, 2014 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 6
Basım Tarihi: 2014
Doi Numarası: 10.1093/gbe/evu263
Dergi Adı: GENOME BIOLOGY AND EVOLUTION
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.3281-3294
Anahtar Kelimeler: pneumococcal isolates, whole-genome sequencing, comparative genomics, integrative conjugative elements, antibiotic nonsusceptibility, CONJUGATIVE ELEMENTS, RESISTANCE, GENE, COLONIZATION, PNEUMOCOCCI, EVOLUTION, CONJUNCTIVITIS, PREVALENCE, EXPRESSION, VIRULENCE
Hacettepe Üniversitesi Adresli: Evet

Özet

The surrounding capsule of Streptococcus pneumoniae has been identified as a major virulence factor and is targeted by pneumococcal conjugate vaccines (PCV). However, nonencapsulated S. pneumoniae (non-Ec-Sp) have also been isolated globally, mainly in carriage studies. It is unknown if non-Ec-Sp evolve sporadically, if they have high antibiotic nonsusceptiblity rates and a unique, specific gene content. Here, whole-genome sequencing of 131 non-Ec-Sp isolates sourced from 17 different locations around the world was performed. Results revealed a deep-branching classic lineage that is distinct from multiple sporadic lineages. The sporadic lineages clustered with a previously sequenced, global collection of encapsulated S. pneumoniae (Ec-Sp) isolates while the classic lineage is comprised mainly of the frequently identified multilocus sequences types (STs) ST344 (n = 39) and ST448 (n= 40). All ST344 and nine ST448 isolates had high nonsusceptiblity rates to beta-lactams and other antimicrobials. Analysis of the accessory genome reveals that the classic non-Ec-Sp contained an increased number of mobile elements, than Ec-Sp and sporadic non-Ec-Sp. Performing adherence assays to human epithelial cells for selected classic and sporadic non-Ec-Sp revealed that the presence of a integrative conjugative element (ICE) results in increased adherence to humanepithelial cells (P= 0.005). Incontrast, sporadic non-EcSp lacking the ICE had greater growth in vitro possibly resulting in improved fitness. In conclusion, non-Ec-Sp isolates from the classic lineage have evolved separately. They have spread globally, are well adapted to nasopharyngeal carriage and are able to coexist with Ec-Sp. Due to continued use of PCV, non-Ec-Sp may become more prevalent.