Antisense oligonucleotide delivery to cancer cell lines for the treatment of different cancer types


KILIÇAY E., Erdal E., HAZER B., TÜRK M., DENKBAŞ E. B.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.44, ss.1938-1948, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 44 Konu: 8
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3109/21691401.2015.1115409
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Sayfa Sayıları: ss.1938-1948

Özet

Amphiphilic poly(3-hydroxylalkanoate) (PHA) copolymers find interesting applications in drug delivery. The aim of this study was to prepare nucleic acid adsorbed on (PHB-b-PEG-NH2) nanoparticle platform for gene delivery. For this purpose, PHB-b-PEG-NH2 block copolymers were synthesized via transesterification reactions. The copolymers obtained were characterized by Proton Nuclear Magnetic Resonance (H-1-NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) techniques. The cytotoxic, apoptotic and necrotic effects of these nanoparticles in the MDA 231 human breast cancer cell, the A549 human lung cancer cell and the L929 fibroblast cell lines were also investigated.