Several Tick-Borne Pathogenic Viruses in Circulation in Anatolia, Turkey


DİNÇER E., TİMURKAN M. Ö. , Oğuz B., Sahindokuyucu I., Sahan A., Ekinci M., ...More

VECTOR-BORNE AND ZOONOTIC DISEASES, vol.22, pp.148-158, 2022 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22
  • Publication Date: 2022
  • Doi Number: 10.1089/vbz.2021.0082
  • Journal Name: VECTOR-BORNE AND ZOONOTIC DISEASES
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.148-158
  • Keywords: tick-borne, Crimean-Congo hemorrhagic fever virus, Jingmen tick virus, Tacheng tick virus, Anatolia, Turkey, HEMORRHAGIC-FEVER VIRUS, FLAVIVIRUS, INFECTION, MOSQUITOS, ALIGNMENT, CHINA

Abstract

Introduction: We screened host-collected ticks for tick-borne viruses, including those recently documented as human pathogens.Methods: During 2020-2021, ticks removed form cattle, sheep, dogs, and cats in 11 provinces in 5 geographically distinct regions of Anatolia were identified, pooled, and screened using pan-nairovirus, pan-flavivirus and individual assays for Jingmen tick virus (JMTV), and Tacheng tick virus 1 and 2 (TcTV-1 and TcTV-2).Results: A total of 901 tick specimens, comprising 6 species were included. Rhipicephalus sanguineus complex was the most abundant species (44.1%), followed by Rhipicephalus bursa (38.3%), Haemaphysalis parva (7.2%), and others. The specimens were screened in 158 pools with 12 pools (7.6%) being positive. Crimean-Congo hemorrhagic fever virus (CCHFV) lineage Europe 2 (genotype VI) sequences were detected in R. bursa in five (3.2%) of the pools, with similar prevalences in central and Mediterranean Anatolian provinces. JMTV was identified in four R. bursa and one Rhipicephalus turanicus pools, collected from Mediterranean and southeastern Anatolia, with a CCHFV and JMTV coinfected R. bursa pool. The JMTV segment 1 sequences formed a separate cluster with those from Turkey and the Balkan peninsula in the maximum likelihood analysis. TcTV-2 was detected in two Dermacentor marginatus specimens (1.3%) collected in central Anatolia, with nucleocapsid sequences forming a phylogenetically segregated group among viruses from humans and ticks from China and Kazakhstan.Discussion: CCHFV Europe 2 was initially documented in ticks from central Anatolian locations, where related orthonairoviruses had been previously recorded. Ongoing activity and a wider distribution of JMTV and TcTV-2 were observed. These viruses should be screened as potential etiological agents in human infections associated with tick bites.