Establishment of Green Fluorescent Protein Expressing CHO Cells by Stable Transfection Using Activated Dendrimers and G418 Selection

CELTIKCI B., PURALI N., ÖZKARA H. A.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.35, no.4, pp.340-343, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 4
  • Publication Date: 2010
  • Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.340-343
  • Hacettepe University Affiliated: Yes

Abstract

Background. Stable transfection of Green Fluorescent Protein (GFP) has been widely used as a marker for both monitoring protein expression and direct observations of cellular dynamics. Despite the proven utility of GFP as a marker, the appropriate conditions of stable transfection, like selection of optimal concentration of G418 and the effect of passage number on fluorescence emission time has not been fully described for stable transfection of kanamycin/neomycin resistant pIRES-EGFP to Chinese Hamster Ovary (CHO) cells by activated dendrimers.