Improving Microcirculatory Reperfusion Reduces Parenchymal Oxygen Radical Formation and Provides Neuroprotection.


TASKIRAN-SAG A., YEMISCI M., GURSOY-OZDEMIR Y., ERDENER Ş. E. , KARATAS H., YÜCE D., ...More

Stroke, vol.49, no.5, pp.1267-1275, 2018 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 5
  • Publication Date: 2018
  • Doi Number: 10.1161/strokeaha.118.020711
  • Journal Name: Stroke
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.1267-1275
  • Keywords: animals, ischemia, neuroprotection, nitrones, no-reflow phenomenon, reactive oxygen species, reperfusion injury, ACUTE ISCHEMIC-STROKE, FOCAL CEREBRAL-ISCHEMIA, SUPEROXIDE-DISMUTASE DEFICIENCY, BUTYL-NITRONE PBN, LASER SPECKLE, S-PBN, BLOOD-FLOW, NXY-059, BRAIN, RATS

Abstract

Background and Purpose-Reperfusion is the most significant determinant of good outcome after ischemic stroke. However, complete reperfusion often cannot be achieved, despite satisfactory recanalization. We hypothesized that microvascular protection was essential for achieving effective reperfusion and, hence, neuroprotection. To test this hypothesis, we have developed an in vivo model to differentially monitor parenchymal and vascular reactive oxygen species (ROS) formation. By comparing the ROS-suppressing effect of N-tert-butyl-alpha-phenylnitrone (PBN) with its blood-brain barrier impermeable analog 2-sulfo-phenyl-N-tert-butylnitrone (S-PBN), we assessed the impact of vascular ROS suppression alone on reperfusion and stroke outcome after recanalization.