Research Information System
Current Topics in Medicinal Chemistry, 2026 (SCI-Expanded, Scopus)
Introduction: Glioblastoma multiforme is one of the common central nervous system tumors characterized by a low survival rate and a high risk of recurrence. This study aimed to investigate the cell viability and ER stress in the T98G glioma cell line after the application of Biochanin A, an isoflavone. Methods: A non-toxic dose was determined for T98G cells after Biochanin A application. Then, using a non-toxic concentration of Biochanin A, a flow cytometry experiment was performed for apoptotic cell ratios, quantitative Real-time PCR was performed to verify the expression levels of GRP78, ATF4, EIF2S1, and XBP1 genes that are associated with ER stress, a wound healing assay was performed to show cellular migration, and intracellular Ca2+ levels were determined. Results: The ratio of early and late apoptosis was low for the control group compared to Biochanin A-treated cells. Although it was not statistically significant, a trend towards increased apoptosis was observed after Biochanin A application. Biochanin A-treated T98G cells showed reduced migration compared to control cells. Among the studied genes, ATF4 was statistically upregulated (p=0.0002) while no changes in intracellular Ca2+ level were observed. Discussion: In this study, the effects of Biochanin A application on glioblastoma cells were evaluated from the perspective of cell viability and ER stress. We concluded that Biochanin A application upregulates the ATF4 gene expression, which has important roles during tumor progression and affects the migration of T98G glioblastoma cells. Conclusion: The results of the study will contribute to the development of new treatment strategies supported by isoflavones such as Biochanin A.