Preparation of imprinted cryogel cartridge for chiral separation of l-phenylalanine

AKGÖNÜLLÜ S., Yavuz H., DENİZLİ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, cilt.45, sa.4, ss.800-807, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 4
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/21691401.2016.1175445
  • Dergi Adı: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.800-807
  • Anahtar Kelimeler: Affinity cryogel, amino acid imprinting, chiral separation, l-phenylalanine, PHEMA, PERFORMANCE LIQUID-CHROMATOGRAPHY, METHACRYLATE) BASED CRYOGEL, AROMATIC-AMINO-ACIDS, SUPERMACROPOROUS CRYOGELS, CAPILLARY ELECTROCHROMATOGRAPHY, ENANTIOSELECTIVE SEPARATION, ALBUMIN DEPLETION, EMBEDDED CRYOGELS, BETA-CYCLODEXTRIN, HUMAN SERUM
  • Hacettepe Üniversitesi Adresli: Evet

Özet

l-Phe-imprinted cryogel cartridge was prepared for the chiral separation of l-Phe. N-Methacryloyl l-phenylalanine (MAPA) was used as a functional monomer for complexing with l-Phe. The selectivity of the membranes was investigated by using d-Phe, l-Trp, and d-Trp as competitor molecules. The PHEMAPA-l-Trp membranes were 6.4, 4.3, and 5.5 times more selective for l-Phe than d-Phe, l-Trp, and d-Trp, respectively. The PHEMAPA-l-Phe cryogel cartridge was incorporated into the fast protein liquid chromatography (FPLC) equipment and was able to separate D,l-Phe racemic mixture efficiently. The PHEMAPA-l-Phe membranes were shown to be reusable many times without significant loss of the adsorption capacity.