Preparation of imprinted cryogel cartridge for chiral separation of l-phenylalanine

AKGÖNÜLLÜ S., Yavuz H., DENİZLİ A.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.45, no.4, pp.800-807, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.1080/21691401.2016.1175445
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.800-807
  • Keywords: Affinity cryogel, amino acid imprinting, chiral separation, l-phenylalanine, PHEMA, PERFORMANCE LIQUID-CHROMATOGRAPHY, METHACRYLATE) BASED CRYOGEL, AROMATIC-AMINO-ACIDS, SUPERMACROPOROUS CRYOGELS, CAPILLARY ELECTROCHROMATOGRAPHY, ENANTIOSELECTIVE SEPARATION, ALBUMIN DEPLETION, EMBEDDED CRYOGELS, BETA-CYCLODEXTRIN, HUMAN SERUM
  • Hacettepe University Affiliated: Yes

Abstract

l-Phe-imprinted cryogel cartridge was prepared for the chiral separation of l-Phe. N-Methacryloyl l-phenylalanine (MAPA) was used as a functional monomer for complexing with l-Phe. The selectivity of the membranes was investigated by using d-Phe, l-Trp, and d-Trp as competitor molecules. The PHEMAPA-l-Trp membranes were 6.4, 4.3, and 5.5 times more selective for l-Phe than d-Phe, l-Trp, and d-Trp, respectively. The PHEMAPA-l-Phe cryogel cartridge was incorporated into the fast protein liquid chromatography (FPLC) equipment and was able to separate D,l-Phe racemic mixture efficiently. The PHEMAPA-l-Phe membranes were shown to be reusable many times without significant loss of the adsorption capacity.