Introduction Care teams in nursing homes have to meet the expectations of the people they care for, their relatives and the institutions. This affects the quality of the care. An increased care burden may negatively affect the quality of work, lives and the health of formal caregivers. Objectives This methodological cross-sectional study was conducted to adapt the Professional Care Team Burden (PCTB) Scale, which was developed to assess the difficulties experienced by caregivers working in long-term care, to Turkish and test its validity and reliability. Material and method The study was conducted with 100 caregivers working at a nursing home and rehabilitation centre. The Caregiver Information Form and the Professional Care Team Burden Scale were used to collect data. The language and content validity, construct validity and reliability of the Turkish version of the scale were tested, respectively. Results The factor analysis showed that the Professional Care Team Burden Scale could be used in two dimensions. When the test-retest was done, the reliability of the scale was found to be high (0.982). The construct validity of the scale showed that the three-factor model did not acceptably fit the data, while the two-dimensional model obtained by removing some of the variables from the model did fit the data (RMSEA = 0.106, GFI = 0.933, CFI = 0.916, IFI = 0.919, RFI = 0.778 and RMR = 0.078). The factor loadings for the construct validity of the scale demonstrated that the two-factor model showed an acceptable fit. Conclusions The study found that the two-factor Turkish version of the Professional Care Team Burden Scale met the validity and reliability criteria. The scale, which has an important place in the assessment of the formal caregivers' burden, can be used with healthcare professionals providing care for older adults.