Light and Electron Microscopic Examination of Exocrine Pancreas Using Zinc Iodide-Osmium Tetroxide Technique

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ATİLLA P., Akkuş M., Deveci E., Bilgin Y., Inalöz S., Daǧdeviren A.

Okajimas Folia Anatomica Japonica, vol.75, no.5, pp.247-250, 1998 (Scopus) identifier identifier


Zinc iodide-osmium tetroxide (ZIO) fixation/staining technique is a metallophilic technique which has been used for the examination of various tissues and cell types. We examined the ZIO (+) cell types in rat exocrine pancreas to obtain further evidence for the significance of the reaction. Among mostly non-reactive pancreatic acinar cells there were ZIO (+) acinar cells of varying staining intensity. Zymogenic granules and centroacinar cells were completely non-reactive. Our electron microscopic findings support the view that the reactivity of the technique used is cell specific but not cell type or organelle specific.