Light and Electron Microscopic Examination of Exocrine Pancreas Using Zinc Iodide-Osmium Tetroxide Technique

ATİLLA P. , Akkuş M., Deveci E., Bilgin Y., Inalöz S., Daǧdeviren A.

Okajimas Folia Anatomica Japonica, vol.75, no.5, pp.247-250, 1998 (Refereed Journals of Other Institutions) identifier identifier

  • Publication Type: Article / Article
  • Volume: 75 Issue: 5
  • Publication Date: 1998
  • Doi Number: 10.2535/ofaj1936.75.5_247
  • Title of Journal : Okajimas Folia Anatomica Japonica
  • Page Numbers: pp.247-250


Zinc iodide-osmium tetroxide (ZIO) fixation/staining technique is a metallophilic technique which has been used for the examination of various tissues and cell types. We examined the ZIO (+) cell types in rat exocrine pancreas to obtain further evidence for the significance of the reaction. Among mostly non-reactive pancreatic acinar cells there were ZIO (+) acinar cells of varying staining intensity. Zymogenic granules and centroacinar cells were completely non-reactive. Our electron microscopic findings support the view that the reactivity of the technique used is cell specific but not cell type or organelle specific.