Beauvericin purification from fungal strain using molecularly imprinted cryogels


ULUSOY M., ASLIYÜCE ÇOBAN S., KESKİN N., DENİZLİ A.

PROCESS BIOCHEMISTRY, vol.113, pp.185-193, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 113
  • Publication Date: 2022
  • Doi Number: 10.1016/j.procbio.2021.12.031
  • Journal Name: PROCESS BIOCHEMISTRY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Communication Abstracts, Compendex, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.185-193
  • Keywords: Molecular imprinting technology, Beauvericin, Cryogel, Purification, ENTOMOPATHOGENIC FUNGI, PROTEIN, METABOLITES, APOPTOSIS, ENNIATINS, BASSIANA, PERSPECTIVES, INHIBITORS, INDUCTION, TOXICITY

Abstract

Beauveria bassiana is a wide host range entomopathogenic fungus that plays a substantial part to control of insect populations. During its pathogenic phase, fungus syntheses several metabolites. One of these metabolites is beauvericin (BEA) and it shows as an antibiotic, antifungal, insecticidal activity. Molecular imprinting approach allows selective and sensitive recognition of chosen template molecule by leaving artificial imprinted cavities in polymer matrix that provides high affinity and selectivity toward desired compound. Cryogels are gel matrices prepared by the method of cryotropic gelation performed below the freezing point of monomer solutions. Under these special conditions, the polymer material resulting from gel formation has rather unusual macroporous morphologies and processability. In this study, BEA-imprinted cryogel columns were prepared for purification of beauvericin from fungal extracts. According to the experimental results, optimum adsorption conditions were determined as pH 6.5 buffer solution, 1 mL/min flow rate and 25 degrees C. Under these conditions, the highest adsorption capacity of cryogels was found to be 43 mg/g. The adsorption isotherm is consistent with the Freundlich adsorption isotherm. In addition, BEA imprinted cryogels are 9.5 times more selective against the template molecule BEA than bassiatin, which is the competitive agent.