In this study, the effect of crosslinking conditions was investigated to obtain insoluble poly(ethylene oxide) (PEO) nanofiber mats having an ultraviolet (UV) initiating and crosslinking agent, pentaerythritol triacrylate (PETA), with various ratios in the presence or absence of UV irradiation at 366 nm. At first, PEO nanofibers were electrospun from 400,000 and 600,000 g/mole molecular weights of PEO and they were compared in terms of diameter and fiber morphology. Whereas applied voltage in the range of 1025 kV had no significant effect on the fiber morphology, fiber diameters varied by voltage. An increase in the flow rate from 0.25 to 1.00 mL h(-1) had an effect in favor of fabricating thicker fibers. The effect of distance to collector on the diameter and morphology was not distinctive. Fibers having irregular morphology and beads appeared with increasing the polymer concentration from 4 to 8 % w/v. Prior crosslinking, electrospinning process at selected conditions was applied to the PEO (600,000) including PETA and PEO-PETA nanofibers were obtained. Besides PETA concentration and UV application, drying conditions before UV irradiation were also found effective to obtain stable fibers in aqueous media. PEO nanofibers electrospun in the presence of 10 % PETA (w/w), dried for 8 days at 37 degrees C in an air atmosphere and then, irradiated with UV for 50 min were found most stable in aqueous media. However, crosslinking was also achieved in the absence of UV.