Purification and characterization of polyhydroxyalkanoate (PHA) from a Bacillus megaterium strain using various dehydration techniques

AKDOĞAN M., Celik E.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol.93, no.8, pp.2292-2298, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 93 Issue: 8
  • Publication Date: 2018
  • Doi Number: 10.1002/jctb.5572
  • Journal Name: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2292-2298
  • Keywords: polyhydroxyalkanoate, poly(3-hydroxybutyrate), bacterial biopolymer, lyophilization, microwave-assisted, purification, RALSTONIA-EUTROPHA, WASTE-WATER, NILE RED, RECOVERY, POLY-3-HYDROXYBUTYRATE, POLY(3-HYDROXYBUTYRATE), EXTRACTION, BIOSYNTHESIS, BACTERIA
  • Hacettepe University Affiliated: Yes

Abstract

BACKGROUNDPolyhydroxyalkanoates (PHAs) are natural biodegradable polymers synthesized by several microorganisms. Poly(3-hydroxybutyrate) (PHB) is the most well-characterized biopolymer produced abundantly by Bacillus species. In order to get a better PHA recovery, efficient pretreatment steps, especially dehydration, have a large impact on PHA purification from microbial PHA-rich biomass. In this study, we have described the effects of lyophilization (-L), microwave-assisted drying (-M) and ethanol/heat-treatment (-E) dehydration techniques on PHA production by B. megaterium NRRL B-14308 strain.