Purification and characterization of polyhydroxyalkanoate (PHA) from a Bacillus megaterium strain using various dehydration techniques
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, cilt.93, sa.8, ss.2292-2298, 2018 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 93 Sayı: 8
- Basım Tarihi: 2018
- Doi Numarası: 10.1002/jctb.5572
- Dergi Adı: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2292-2298
- Anahtar Kelimeler: polyhydroxyalkanoate, poly(3-hydroxybutyrate), bacterial biopolymer, lyophilization, microwave-assisted, purification, RALSTONIA-EUTROPHA, WASTE-WATER, NILE RED, RECOVERY, POLY-3-HYDROXYBUTYRATE, POLY(3-HYDROXYBUTYRATE), EXTRACTION, BIOSYNTHESIS, BACTERIA
- Hacettepe Üniversitesi Adresli: Evet
Özet
BACKGROUNDPolyhydroxyalkanoates (PHAs) are natural biodegradable polymers synthesized by several microorganisms. Poly(3-hydroxybutyrate) (PHB) is the most well-characterized biopolymer produced abundantly by Bacillus species. In order to get a better PHA recovery, efficient pretreatment steps, especially dehydration, have a large impact on PHA purification from microbial PHA-rich biomass. In this study, we have described the effects of lyophilization (-L), microwave-assisted drying (-M) and ethanol/heat-treatment (-E) dehydration techniques on PHA production by B. megaterium NRRL B-14308 strain.