The control of the agricultural pests is crucial for commercial products. There is a growing interest for biological control of these pests and one of the most effective and safe biocontrol agents is Beauveria bassiana (Balsamo-Crivelli) Vuillemin, which is an entomopathogenic fungus. The soil environment is a significant resource for entomopathogenic fungi. In this study the diversity and distribution of entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. was assessed throughout an extensive soil survey in Turkey carried out during 2013 and 2014. Soil samples from the seven regions of Turkey were taken and Beauveria spp. isolates were obtained from six of these regions, with 32 positive sites out of 150 sites sampled. The isolates were identified as Beauveria bassiana based on phenotypic and molecular data. Beauveria bassiana was isolated from the European (Marmara) as well as the Asiatic peninsula (Anatolia) of Turkey.