Purpose To investigate if chewing side preference (CSP) can be used as an indicator of hemispheric laterality in healthy adults. Materials and methods Seventy-five individuals were included. The visual analogue scale (VAS) was used to determine CSP and laterality test for preferred peripheral organs. Results Significant correlation between CSP and hand, foot, ear, and eye side preference was found (r=.41, p<.001; r=.34, p=.003; r=.35, p=.03; r=.36, p=.002). Conclusion Besides peripheral organs, the CSP can also be used in determination of hemispheric lateralization.